Proteins in Hours
Advancements in the field of biopharmaceuticals and protein engineering necessitate the development of new methods for on-demand production of chemically modified proteins and peptides. Standard solid-phase peptide synthesis is currently not routinely capable of producing peptides over 50 amino acids in length, and proteins are typically obtained by ligation techniques and recombinant expression.
At the American Peptide Symposium 2019, Nina Hartrampf from the Pentelute Group at Massachusetts Institute of Technology, winner of a 2019 Young Investigators Oral Presentation award, reported on a total synthesis methodology that bridges the gap between midsized peptides and single domain proteins. These findings are based on an automated flow peptide synthesis, AFPS, platform developed in the Pentelute lab in 2017.
Using AFPS, they are now able to routinely produce peptide sequences exceeding 50 residues in length. Fully functional protein molecules including HIV-1 protease, bacterial RNAse, and the oncogenic ubiquitin ligase MDM2 were synthesized by this method in multimilligram quantities and with unprecedented speed.